Root salt stress treatment
  • G_C: 600 mM NaCl root treatment for 0 h
  • G_T1: 600 mM NaCl root treatment for 3 h
  • G_T2: 600 mM NaCl root treatment for 24 h
  • G_T3: 600 mM NaCl root treatment for 3 d
  • G_T4: 600 mM NaCl root treatment for 7 d
Leaf salt stress treatment
  • L_C: 600 mM NaCl leaf treatment for 0 h
  • L_T1: 600 mM NaCl leaf treatment for 3 h
  • L_T2: 600 mM NaCl leaf treatment for 24 h
  • L_T3: 600 mM NaCl leaf treatment for 3 d
  • L_T4: 600 mM NaCl leaf treatment for 7 d
Different tissues
  • Sep: Sepals
  • Pet: Petals
  • Sty: Stigma
  • Cap: Carpels
  • Ova: Ovary
  • Ant: Anthers
  • Fil: Filaments
  • Rec: Receptacle
  • Ped: Peduncle
Temperature stress treatment
  • The seeds of Ipc were collected from the seashore of Changle, Fuzhou (latitude 26°, 5′, 16′ N, longitude 119°, 14′, 6′ E), Fujian, China. The seed coats were cut with scissors at the corner to make sure the water could enter. The seeds were then soaked in water at 37℃ for 48 h. The germinated seedlings were transformed to soil and planted in the greenhouse (light cycle: 16 h/8 h day/night cycle; light intensity: 200 μmol m − 2 s − 1; relative humidity: 30%–50%; temperature: 25℃). Ninety days after germination, the plants were divided into nine groups (3 treatments, 3 biological groups for each treatment) and transferred to the separate growth chambers with the temperature degrees set to 12℃, 25℃, and 40℃, respectively. After ten days of growth in the growth chambers, the top 3–6 leaves of Ipc were collected for RNA isolation.
  • 25℃ Temperature control group: CK_1, CK_2, CK_3
  • 12℃ Cold treatment group: Cold_1, Cold_2, Cold_3
  • 40℃ Heat treatment group: Heat_1, Heat_2, Heat_3
RNA-seq analysis data
1 TPM_matrix
2 DEG_CK_vs_Cold
3 DEG_CK_vs_Heat
4 The enriched GOs with DEGs in root under time-course salt-treatment
5 The enriched GOs with DEGs in leaf under time-course salt-treatment
6 Summary of GO enrichment result in root and leaves of IPC under salt-treatment
7 Time-course salt-treatment analysis provides insight into the salt tolerance of IPC at the transcriptional level
References
  1. Cheng Y#, Wang Y, Sun J, et al. (2023). Unveiling the genomic blueprint of salt stress: insights from Ipomoea pes-caprae L. Seed Biology. doi:10.48130/SeedBio-2023-0021.
  2. Cheng Y#, Zhou Q#, Li W, et al. (2021). De novo transcriptome assembly and gene expression profiling of Ipomoea pes-caprae L. under heat and cold stresses. Scientia Horticulturae, 289, 110379.
  3. Liu Y, Dai X, Zhao L, et al. (2020). RNA-seq reveals the salt tolerance of Ipomoea pes-caprae, a wild relative of sweet potato. Journal of Plant Physiology, 255, 153276.